The effects of nitrogen pollutants on the isotopic signal (δ15N) of Ulva lactuca. Microcosm experiments

Effects of two chemical forms of Nitrogen (NH4 + and NO3 −) on δ15N in Ulva lactuca were analysed separately and in mixture at two concentrations.We assessedwhether the δ15Nvaluesof U. lactuca discriminate between Nitrogen from synthetic fertilisers (inorganic) and from fresh cow manure (organic), and the isotopic ability of the macroalga to reflect Nitrogen concentrations. Isotopic signature and N content of themacroalga reflected different nitrogenous sources and their concentrations after 48 h. The inorganic Nitrogen source (NH4NO3) altered the isotopic values of themacroalgaemore than Nitrogen fromfresh cowmanure (NO3 −). δ15N values observed in the mixed solution did not differ from those displayed in NH4NO3 treatment alone.We conclude that stable isotope analysis of U. lactuca collected in an unpolluted site and experimentally submerged in sites suspected of being affected by disturbance is a useful tool for rapid monitoring of anthropogenic discharges of Nitrogen pollutants.